ALDH3A1 Mouse Monoclonal Antibody [Clone ID: OTI1B6]

CAT#: CF501105

Carrier-free (BSA/glycerol-free) ALDH3A1 mouse monoclonal antibody, clone OTI1B6 (formerly 1B6)

Product Images

HEK293T cells transfected with either pCMV6-ENTRY ALDH3A1 (RC202440) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ALDH3A1 mouse monoclonal (TA501105), and then analyzed by flow cytometry.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A1 (RC202440, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A1. Positive lysates LY400232 (100ug) and LC400232 (20ug) can be purchased separately from OriGene.

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  Anti-ALDH3A1 mouse monoclonal antibody (TA501105) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH3A1 (RC202440).

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501105, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501105, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501105, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501105, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ALDH3A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501105, Dilution 1:50)

• Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-ALDH3A1 monoclonal antibody at 1:200 dilution.

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Specifications

Product Data

• Clone Name: OTI1B6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALDH3A1(NP_000682) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.2 kDa
• Gene Name: Homo sapiens aldehyde dehydrogenase 3 family member A1 (ALDH3A1), transcript variant 2, mRNA.
• Database Link:
  NP_000682
  Entrez Gene 218 Human
  UniProt ID P30838
• Background: Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Synonyms: ALDH3; ALDHIII

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism