BTK Mouse Monoclonal Antibody [Clone ID: OTI10E10]

CAT#: CF501118

Carrier-free (BSA/glycerol-free) BTK mouse monoclonal antibody, clone OTI10E10 (formerly 10E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (RC211582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK. Positive lysates LY424947 (100ug) and LC424947 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BTK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501118, Dilution 1:50)

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  Flow cytometric analysis of living 293T cells transfected with BTK overexpression plasmid (RC211582), Red)/empty vector (PS100001, Blue) using anti-BTK antibody (TA501118). Cells incubated with a non-specific antibody (Green) were used as isotype control.

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  Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody (TA501118).

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  Anti-BTK mouse monoclonal antibody (TA501118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK (RC211582).

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BTK monoclonal antibody at 1:200 dilution.

• Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BTK monoclonal antibody (1:200).

• Figure from citation: Flow Cytometry analysis of intracellular BTK expression by using anti-BTK antibody (clone OTI10E10) in CD14+ monocytes. Black histograms indicate BTK expression and Gray histograms indicate isotype control. BTK expression in the patient was reduced compared with that in a normal control. View Citation

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Specifications

Product Data

• Clone Name: OTI10E10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 76.3 kDa
• Gene Name: Homo sapiens Bruton tyrosine kinase (BTK), transcript variant 1, mRNA.
• Database Link:
  NP_000052
  Entrez Gene 12229 MouseEntrez Gene 367901 RatEntrez Gene 695 Human
  UniProt ID Q06187
• Background: The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement.
• Synonyms: AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency