RhoGDI (ARHGDIA) Mouse Monoclonal Antibody [Clone ID: OTI1A7]

CAT#: CF501120

Carrier-free (BSA/glycerol-free) ARHGDIA mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (RC200902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA. Positive lysates LY401371 (100ug) and LC401371 (20ug) can be purchased separately from OriGene.

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  Anti-ARHGDIA mouse monoclonal antibody (TA501120) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGDIA (RC200902).

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-ARHGDIA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ARHGDIA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ARHGDIA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Equivalent amounts of cell lysates (30 ug per lane) of wild-type HAP-1 cells(WT) and ARHGDIA-Knockdown HAP-1 cells(KD) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA monoclonal antibody TA501120 (1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

• Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and ARHGDIA-Knockout 293T cells (KO, Cat# LC811309) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA monoclonal antibody TA501120, (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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Specifications

Product Data

• Clone Name: OTI1A7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARHGDIA(NP_004300) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23 kDa
• Gene Name: Rho GDP dissociation inhibitor alpha
• Database Link:
  NP_004300
  Entrez Gene 192662 MouseEntrez Gene 360678 RatEntrez Gene 396 Human
  UniProt ID P52565
• Background: Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA
• Synonyms: GDIA1; HEL-S-47e; NPHS8; RHOGDI; RHOGDI-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Neurotrophin signaling pathway