DNTTIP1 Mouse Monoclonal Antibody [Clone ID: OTI4F7]

CAT#: CF501130

Carrier-free (BSA/glycerol-free) DNTTIP1 mouse monoclonal antibody, clone OTI4F7 (formerly 4F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNTTIP1 (RC206867, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNTTIP1. Positive lysates LY403275 (100ug) and LC403275 (20ug) can be purchased separately from OriGene.

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  Anti-DNTTIP1 mouse monoclonal antibody (TA501130) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNTTIP1 (RC206867).

Specifications

Product Data

• Clone Name: OTI4F7
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNTTIP1 (NP_443183) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.8 kDa
• Gene Name: Homo sapiens deoxynucleotidyltransferase terminal interacting protein 1 (DNTTIP1), mRNA.
• Database Link:
  NP_443183
  Entrez Gene 76233 MouseEntrez Gene 171437 RatEntrez Gene 116092 Human
  UniProt ID Q9H147
• Background: DNTTIP1 binds DNA and enhances the activity of terminal deoxynucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template
• Synonyms: C20orf167; dJ447F3.4; Tdif1