CDHH (CDH13) Mouse Monoclonal Antibody [Clone ID: OTI3H6]

CAT#: CF501158

Carrier-free (BSA/glycerol-free) CDH13 mouse monoclonal antibody, clone OTI3H6 (formerly 3H6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH13 (RC206068, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH13. Positive lysates LY400505 (100ug) and LC400505 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CDH13 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Anti-CDH13 mouse monoclonal antibody (TA501158) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDH13 (RC206068).

Specifications

Product Data

• Clone Name: OTI3H6
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDH13 (NP_001248) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.9 kDa
• Gene Name: Homo sapiens cadherin 13 (CDH13), transcript variant 1, mRNA.
• Database Link:
  NP_001248
  Entrez Gene 12554 MouseEntrez Gene 192248 RatEntrez Gene 1012 Human
  UniProt ID P55290
• Background: This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites
• Synonyms: CDHH; P105