TUBA8 Mouse Monoclonal Antibody [Clone ID: OTI3G2]

CAT#: CF501159

Carrier-free (BSA/glycerol-free) TUBA8 mouse monoclonal antibody, clone OTI3G2 (formerly 3G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8. Positive lysates LY412867 (100ug) and LC412867 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3G2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.9 kDa
• Gene Name: Homo sapiens tubulin alpha 8 (TUBA8), transcript variant 1, mRNA.
• Database Link:
  NP_061816
  Entrez Gene 500377 RatEntrez Gene 51807 Human
  UniProt ID Q9NY65
• Background: This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: TUBAL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection