CD147 (BSG) Mouse Monoclonal Antibody [Clone ID: OTI9H5]

CAT#: CF501164

Carrier-free (BSA/glycerol-free) BSG mouse monoclonal antibody, clone OTI9H5 (formerly 9H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BSG (RC219464, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BSG. Positive lysates LY419777 (100ug) and LC419777 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BSG monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BSG monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and BSG-Knockout 293T cells (KO, Cat#LC810433) were separated by SDS-PAGE and immunoblotted with anti-BSG monoclonal antibody TA501164 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501164, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501164, Dilution 1:50)

• Anti-BSG mouse monoclonal antibody (TA501164) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BSG (RC219464).

• Immunofluorescent staining of HT29 cells using anti-BSG mouse monoclonal antibody (TA501164).

• HEK293T cells transfected with either RC219464 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BSG antibody (TA501164), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9H5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1500~3000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.2 kDa
• Gene Name: Homo sapiens basigin (Ok blood group) (BSG), transcript variant 1, mRNA.
• Database Link:
  NP_001719
  Entrez Gene 25246 RatEntrez Gene 476758 DogEntrez Gene 721068 MonkeyEntrez Gene 682 Human
  UniProt ID P35613
• Background: The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: 5F7; CD147; EMMPRIN; EMPRIN; OK; SLC7A11; TCSF

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane