ACSBG1 Mouse Monoclonal Antibody [Clone ID: OTI2B5]

CAT#: CF501175

Carrier-free (BSA/glycerol-free) ACSBG1 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSBG1 (RC203219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSBG1. Positive lysates LY414754 (100ug) and LC414754 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACSBG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501175)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACSBG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501175)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ACSBG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501175)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ACSBG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501175)

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  Anti-ACSBG1 mouse monoclonal antibody (TA501175) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACSBG1 (RC203219).

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Specifications

Product Data

• Clone Name: OTI2B5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACSBG1(NP_055977) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.1 kDa
• Gene Name: Homo sapiens acyl-CoA synthetase bubblegum family member 1 (ACSBG1), transcript variant 1, mRNA.
• Database Link:
  NP_055977
  Entrez Gene 94180 MouseEntrez Gene 171410 RatEntrez Gene 23205 Human
  UniProt ID Q96GR2
• Background: The protein encoded by this gene possesses long-chain acyl-CoA synthetase activity. It is thought to play a central role in brain very long-chain fatty acids metabolism and myelinogenesis. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: BG; BG1; BGM; GR-LACS; LPD