DAP Kinase 2 (DAPK2) Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: CF501204

Carrier-free (BSA/glycerol-free) DAPK2 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Product Images

Anti-DAPK2 mouse monoclonal antibody (TA501204) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DAPK2 (RC216274).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAPK2 (RC216274, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAPK2.

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  Immunoprecipitation (IP) of DAPK2 by using TrueMab monoclonal anti-DAPK2 antibodies (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either RC216274 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAPK2 antibody (TA501204), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-DAPK2 antibody (TA501204), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-DAPK2 antibody (TA501204), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B10
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.7 kDa
• Gene Name: Homo sapiens death associated protein kinase 2 (DAPK2), transcript variant 1, mRNA.
• Database Link:
  NP_055141
  Entrez Gene 13143 MouseEntrez Gene 23604 Human
  UniProt ID Q9UIK4
• Background: This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq]
• Synonyms: DRP-1; DRP1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer