ACAT2 Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CAT#: CF501220

Carrier-free (BSA/glycerol-free) ACAT2 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2. Positive lysates LY417006 (100ug) and LC417006 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ACAT2-Knockout HeLa cells (KO, Cat# LC832703) were separated by SDS-PAGE and immunoblotted with anti-ACAT2 monoclonal antibody TA501220 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501220, Dilution 1:50)

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  Anti-ACAT2 mouse monoclonal antibody (TA501220) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 (RC201821 ).

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  Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody (TA501220).

• HEK293T cells transfected with either RC201821 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACAT2 antibody (TA501220), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1G6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACAT2(NP_005882) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.2 kDa
• Gene Name: Homo sapiens acetyl-CoA acetyltransferase 2 (ACAT2), transcript variant 1, mRNA.
• Database Link:
  NP_005882
  Entrez Gene 308100 RatEntrez Gene 100427660 MonkeyEntrez Gene 39 Human
  UniProt ID Q9BWD1
• Background: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
• Synonyms: acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 2; cytosolic acetoacetyl-CoA thiolase; OTTHUMP00000017527

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Tryptophan metabolism, Valine, leucine and isoleucine degradation