BMAL1 (ARNTL) Mouse Monoclonal Antibody [Clone ID: OTI1D1]

CAT#: CF501229

Carrier-free (BSA/glycerol-free) ARNTL mouse monoclonal antibody, clone OTI1D1 (formerly 1D1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (RC207870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL. Positive lysates LY400474 (100ug) and LC400474 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARNTL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Figure from citation: Immunohistochemistry of ARNTL (BMAL1) protein level by using anti-ARNTL antibody in coronal sections of the main olfactory bulb (MOB) portion of 7-day-old rabbits. Dilution: 1:200 View Citation

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  Anti-ARNTL mouse monoclonal antibody (TA501229) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL (RC207870).

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  HEK293T cells transfected with either RC207870 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARNTL antibody (TA501229), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-ARNTL antibody (TA501229), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1D1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARNTL (NP_001169) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.5 kDa
• Gene Name: Homo sapiens aryl hydrocarbon receptor nuclear translocator like (ARNTL), transcript variant 1, mRNA.
• Database Link:
  NP_001169
  Entrez Gene 11865 MouseEntrez Gene 29657 RatEntrez Gene 406 Human
  UniProt ID O00327
• Background: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal