PTP epsilon (PTPRE) Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CAT#: CF501234

Carrier-free (BSA/glycerol-free) PTPRE mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PTPRE mouse monoclonal antibody. (TA501234; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Anti-PTPRE mouse monoclonal antibody (TA501234) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPRE (RC207950 ).

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  HEK293T cells transfected with either RC207950 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PTPRE antibody (TA501234), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of living Malm3-3m cells, using anti-PTPRE antibody (TA501234), (Red), compared to PBS, (Blue).

Specifications

Product Data

• Clone Name: OTI3H3
• Applications: FC, IF, IHC
• Recommended Dilution: IHC 1:150, IF 1:100, FLOW 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PTPRE (NP_006495) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.5 kDa
• Gene Name: Homo sapiens protein tyrosine phosphatase receptor type E (PTPRE), transcript variant 1, mRNA.
• Database Link:
  NP_006495
  Entrez Gene 19267 MouseEntrez Gene 114767 RatEntrez Gene 5791 Human
  UniProt ID P23469
• Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Two alternatively spliced transcript variants of this gene have been reported, one of which encodes a receptor-type PTP that possesses a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains; Another one encodes a PTP that contains a distinct hydrophilic N-terminus, and thus represents a nonreceptor-type isoform of this PTP. Studies of the similar gene in mice suggested the regulatory roles of this PTP in RAS related signal transduction pathways, cytokines induced SATA signaling, as well as the activation of voltage-gated K+ channels. [provided by RefSeq, Jul 2008]
• Synonyms: HPTPE; PTPE; R-PTP-EPSILON

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transmembrane