RAB17 Mouse Monoclonal Antibody [Clone ID: OTI5E2]

CAT#: CF501239

Carrier-free (BSA/glycerol-free) RAB17 mouse monoclonal antibody, clone OTI5E2 (formerly 5E2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB17 (RC208378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB17. Positive lysates LY402920 (100ug) and LC402920 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RAB17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501239, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RAB17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501239, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RAB17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501239, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RAB17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501239, Dilution 1:50)

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  Anti-RAB17 mouse monoclonal antibody (TA501239) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAB17 (RC208378 ).

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Specifications

Product Data

• Clone Name: OTI5E2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAB17(NP_071894) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.3 kDa
• Gene Name: Homo sapiens RAB17, member RAS oncogene family (RAB17), transcript variant 1, mRNA.
• Database Link:
  NP_071894
  Entrez Gene 64284 Human
  UniProt ID Q9H0T7
• Background: The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is an epithelial cell-specific GTPase
• Synonyms: FLJ12538

Reference Data

• Protein Families: Druggable Genome