SH3PX1 (SNX9) Mouse Monoclonal Antibody [Clone ID: OTI1E4]

CAT#: CF501252

Carrier-free (BSA/glycerol-free) SNX9 mouse monoclonal antibody, clone OTI1E4 (formerly 1E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNX9 (RC202822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX9. Positive lysates LY402520 (100ug) and LC402520 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SNX9 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SNX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501252, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SNX9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501252, Dilution 1:50)

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  Anti-SNX9 mouse monoclonal antibody (TA501252) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNX9 (RC202822 ).

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  Immunofluorescent staining of HeLa cells using anti-SNX9 mouse monoclonal antibody (TA501252).

• HEK293T cells transfected with either RC202822 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNX9 antibody (TA501252), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1E4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNX9(NP_057308) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.4 kDa
• Gene Name: Homo sapiens sorting nexin 9 (SNX9), mRNA.
• Database Link:
  NP_057308
  Entrez Gene 66616 MouseEntrez Gene 683687 RatEntrez Gene 476254 DogEntrez Gene 706016 MonkeyEntrez Gene 51429 Human
  UniProt ID Q9Y5X1
• Background: This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but does contain a SH3 domain near its N-terminus. This protein interacts with the cytoplasmic domains of the precursor but not the processed forms of a disintegrin and metalloprotease domain 9 and 15. This protein binds the beta-appendage domain of adaptor protein 2 and may function to assist adaptor protein 2 in its role at the plasma membrane. This protein interacts with activated Cdc42-associated kinase-2 to regulate the degradation of epidermal growth factor receptor protein.
• Synonyms: SDP1; SH3PX1; SH3PXD3A; WISP

Reference Data

• Protein Families: Druggable Genome