H6PD Mouse Monoclonal Antibody [Clone ID: OTI2A7]

CAT#: CF501257

Carrier-free (BSA/glycerol-free) H6PD mouse monoclonal antibody, clone OTI2A7 (formerly 2A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY H6PD (RC209890, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-H6PD. Positive lysates LY401369 (100ug) and LC401369 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-H6PD monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-H6PD monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501257, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501257, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501257, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-H6PD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501257, Dilution 1:50)

• Anti-H6PD mouse monoclonal antibody (TA501257) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY H6PD (RC209890).

• HEK293T cells transfected with either RC209890 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-H6PD antibody (TA501257), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-H6PD antibody (TA501257), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2A7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:250~500, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human H6PD (NP_004276) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.7 kDa
• Gene Name: Homo sapiens hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase (H6PD), transcript variant 2, mRNA.
• Database Link:
  NP_004276
  Entrez Gene 710107 MonkeyEntrez Gene 9563 Human
  UniProt ID O95479
• Background: There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.
• Synonyms: CORTRD1; G6PDH; GDH

Reference Data

• Protein Pathways: Metabolic pathways, Pentose phosphate pathway