NXNL2 Mouse Monoclonal Antibody [Clone ID: OTI3A12]

CAT#: CF501259

Carrier-free (BSA/glycerol-free) NXNL2 mouse monoclonal antibody, clone OTI3A12 (formerly 3A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NXNL2 (RC205425, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NXNL2. Positive lysates LY408006 (100ug) and LC408006 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NXNL2 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501259, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501259, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501259, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501259, Dilution 1:50)

• Anti-NXNL2 mouse monoclonal antibody (TA501259) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NXNL2 (RC205425 ).

• Flow cytometric Analysis of Jurkat cells, using anti-NXNL2 antibody (TA501259), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Show all 8 images

Specifications

Product Data

• Clone Name: OTI3A12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NXNL2 (NP_660326) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.5 kDa
• Gene Name: Homo sapiens nucleoredoxin like 2 (NXNL2), transcript variant 2, mRNA.
• Database Link:
  NP_660326
  Entrez Gene 75124 MouseEntrez Gene 698533 MonkeyEntrez Gene 158046 Human
  UniProt ID Q5VZ03
• Synonyms: C9orf121; RDCVF2