GNMT Mouse Monoclonal Antibody [Clone ID: OTI8A3]

CAT#: CF501276

Carrier-free (BSA/glycerol-free) GNMT mouse monoclonal antibody, clone OTI8A3 (formerly 8A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNMT (RC207497, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNMT. Positive lysates LY412848 (100ug) and LC412848 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-GNMT monoclonal antibody (1:200).

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  HEK293T cells transfected with either pCMV6-ENTRY GNMT (RC207497) (Red) or empty vector control plasmid (Blue) were immunostained with anti-GNMT mouse monoclonal (TA501276), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI8A3
• Applications: FC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, Flow 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GNMT (NP_061833) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: Homo sapiens glycine N-methyltransferase (GNMT), transcript variant 1, mRNA.
• Database Link:
  NP_061833
  Entrez Gene 27232 Human
  UniProt ID Q14749
• Background: The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia).
• Synonyms: glycine N-methyltransferase; OTTHUMP00000016412

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycine, serine and threonine metabolism