ketohexokinase (KHK) Mouse Monoclonal Antibody [Clone ID: OTI4E9]

CAT#: CF501284

Carrier-free (BSA/glycerol-free) KHK mouse monoclonal antibody, clone OTI4E9 (formerly 4E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (RC202424, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK. Positive lysates LY400082 (100ug) and LC400082 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501284, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501284, Dilution 1:50)

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  Anti-KHK mouse monoclonal antibody (TA501284) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK (RC202424).

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  HEK293T cells transfected with either RC202424 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody (TA501284), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KHK (NP_000212) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.5 kDa
• Gene Name: Homo sapiens ketohexokinase (KHK), transcript variant a, mRNA.
• Database Link:
  NP_000212
  Entrez Gene 3795 Human
  UniProt ID P50053
• Background: This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: ketohexokinase; ketohexokinase (fructokinase)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways