C9orf41 (CARNMT1) Mouse Monoclonal Antibody [Clone ID: OTI6E4]

CAT#: CF501300

Carrier-free (BSA/glycerol-free) C9orf41 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C9orf41 (RC205116, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C9orf41. Positive lysates LY403480 (100ug) and LC403480 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C9orf41 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C9orf41 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501300, Dilution 1:50)

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  Anti-C9orf41 mouse monoclonal antibody (TA501300) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C9orf41 (RC205116).

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  HEK293T cells transfected with either RC205116 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C9orf41 antibody (TA501300), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6E4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C9orf41(NP_689663) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.0 kDa
• Gene Name: Homo sapiens carnosine N-methyltransferase 1 (CARNMT1), transcript variant 1, mRNA.
• Database Link:
  NP_689633
  Entrez Gene 138199 Human
  UniProt ID Q8N4J0
• Synonyms: C9orf41; UPF0586