elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI3H7]

CAT#: CF501314

Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1. Positive lysates LY401321 (100ug) and LC401321 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.

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  HEK293T cells transfected with either RC200368 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EIF2S1 antibody (TA501314), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody (TA501314), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI3H7
• Applications: FC, WB
• Recommended Dilution: WB 1:1000~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.9 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 2 subunit alpha (EIF2S1), mRNA.
• Database Link:
  NP_004085
  Entrez Gene 13665 MouseEntrez Gene 54318 RatEntrez Gene 480361 DogEntrez Gene 710150 MonkeyEntrez Gene 1965 Human
  UniProt ID P05198
• Background: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]). [supplied by OMIM]
• Synonyms: EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A