CD5 Mouse Monoclonal Antibody [Clone ID: OTI6F7]

CAT#: CF501334

Carrier-free (BSA/glycerol-free) CD5 mouse monoclonal antibody, clone OTI6F7 (formerly 6F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. Positive lysates LY415443 (100ug) and LC415443 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA501334) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA501334) (1:500)

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  HEK293T cells transfected with either RC206494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (TA501334), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6F7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: Homo sapiens CD5 molecule (CD5), transcript variant 1, mRNA.
• Database Link:
  NP_055022
  Entrez Gene 695249 MonkeyEntrez Gene 921 Human
  UniProt ID P06127
• Background: CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation (1). CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling (2).
• Synonyms: LEU1; T1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage