CFAP298 Mouse Monoclonal Antibody [Clone ID: OTI2E5]

CAT#: CF501348

Carrier-free (BSA/glycerol-free) C21orf59 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C21orf59 (RC200169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C21orf59. Positive lysates LY402864 (100ug) and LC402864 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C21orf59 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-C21orf59 mouse monoclonal antibody (TA501348) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C21orf59 (RC200169).

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  Flow cytometric Analysis of Jurkat cells, using anti-C21orf59 antibody (TA501348), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI2E5
• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C21orf59 (NP_067077) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.0 kDa
• Gene Name: Homo sapiens cilia and flagella associated protein 298 (CFAP298), transcript variant 1, mRNA.
• Database Link:
  NP_067077
  Entrez Gene 56683 Human
  UniProt ID P57076
• Synonyms: C21orf48; CILD26; FBB18