HIBCH Mouse Monoclonal Antibody [Clone ID: OTI5C5]

CAT#: CF501361

Carrier-free (BSA/glycerol-free) HIBCH mouse monoclonal antibody, clone OTI5C5 (formerly 5C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIBCH (RC209814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIBCH. Positive lysates LY402324 (100ug) and LC402324 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HIBCH monoclonal antibody.

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  Anti-HIBCH mouse monoclonal antibody (TA501361) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HIBCH (RC209814).

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  HEK293T cells transfected with either RC209814 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HIBCH antibody (TA501361), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5C5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HIBCH (NP_055177) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.
• Database Link:
  NP_055177
  Entrez Gene 301384 RatEntrez Gene 607040 DogEntrez Gene 26275 Human
  UniProt ID Q6NVY1
• Background: This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
• Synonyms: HIBYLCOAH

Reference Data

• Protein Pathways: beta-Alanine metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation