INDOL1 (IDO2) Mouse Monoclonal Antibody [Clone ID: OTI1A4]

CAT#: CF501378

Carrier-free (BSA/glycerol-free) IDO2 mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO2 (RC223337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDO2. Positive lysates LY403659 (100ug) and LC403659 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC223337 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IDO2 antibody (TA501378), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-IDO2 antibody (TA501378), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI1A4
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IDO2 (NP_919270) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.9 kDa
• Gene Name: Homo sapiens indoleamine 2,3-dioxygenase 2 (IDO2), mRNA.
• Database Link:
  NP_919270
  Entrez Gene 169355 Human
  UniProt ID Q6ZQW0
• Background: Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]). [supplied by OMIM]
• Synonyms: INDOL1

Reference Data

• Protein Pathways: Metabolic pathways, Tryptophan metabolism