DGKB Mouse Monoclonal Antibody [Clone ID: OTI1F9]

CAT#: CF501381

Carrier-free (BSA/glycerol-free) DGKB mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DGKB (RC211233, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DGKB. Positive lysates LY407898 (100ug) and LC407898 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DGKB monoclonal antibody.

Specifications

Product Data

• Clone Name: OTI1F9
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DGKB (NP_663733) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.9 kDa
• Gene Name: Homo sapiens diacylglycerol kinase beta (DGKB), transcript variant 2, mRNA.
• Database Link:
  NP_663733
  Entrez Gene 54248 RatEntrez Gene 217480 MouseEntrez Gene 482328 DogEntrez Gene 715254 MonkeyEntrez Gene 1607 Human
  UniProt ID Q9Y6T7
• Background: Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
• Synonyms: DAGK2; DGK; DGK-BETA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system