XPNPEP1 Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CAT#: CF501383

Carrier-free (BSA/glycerol-free) XPNPEP1 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Images

HEK293T cells were transfected with pCMV6-ENTRY XPNPEP1 (RC203535, Left lane) or the pCMV6-ENTRY control (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP1.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501383, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501383, Dilution 1:50)

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  Anti-XPNPEP1 mouse monoclonal antibody (TA501383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1 (RC203535).

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Specifications

Product Data

• Clone Name: OTI1E3
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human XPNPEP1 (NP_065116) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.6 kDa
• Gene Name: Homo sapiens X-prolyl aminopeptidase 1 (XPNPEP1), transcript variant 1, mRNA.
• Database Link:
  NP_065116
  Entrez Gene 170750 MouseEntrez Gene 170751 RatEntrez Gene 7511 Human
  UniProt ID Q9NQW7
• Background: This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.
• Synonyms: APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1

Reference Data

• Protein Families: Druggable Genome, Protease