Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: OTI1F3]

CAT#: CF501412

Carrier-free (BSA/glycerol-free) PKLR mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Positive lysates LY400113 (100ug) and LC400113 (20ug) can be purchased separately from OriGene.

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  Anti-PKLR mouse monoclonal antibody (TA501412) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR (RC206455).

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  HEK293T cells transfected with either RC206455 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PKLR antibody (TA501412), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1F3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PKLR (NP_000289) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.6 kDa
• Gene Name: Homo sapiens pyruvate kinase L/R (PKLR), transcript variant 1, mRNA.
• Database Link:
  NP_000289
  Entrez Gene 18770 MouseEntrez Gene 24651 RatEntrez Gene 5313 Human
  UniProt ID P30613
• Background: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: PK1; PKL; PKR; PKRL; RPK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus