mu Crystallin (CRYM) Mouse Monoclonal Antibody [Clone ID: OTI1B5]

CAT#: CF501433

Carrier-free (BSA/glycerol-free) CRYM mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYM (RC218123, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYM. Positive lysates LY419685 (100ug) and LC419685 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-CRYM monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501433, Dilution 1:50)

• 

  Anti-CRYM mouse monoclonal antibody (TA501433) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYM (RC218123).

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:300, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: Homo sapiens crystallin mu (CRYM), mRNA.
• Database Link:
  NP_001879
  Entrez Gene 12971 MouseEntrez Gene 117024 RatEntrez Gene 1428 Human
  UniProt ID Q14894
• Background: Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
• Synonyms: DFNA40; THBP