SHPRH Mouse Monoclonal Antibody [Clone ID: OTI1D12]

CAT#: CF501467

Carrier-free (BSA/glycerol-free) SHPRH mouse monoclonal antibody, clone OTI1D12 (formerly 1D12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (RC212767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Specifications

Product Data

• Clone Name: OTI1D12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 1500-1600 of human SHPRH (NP_775105) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 192.9 kDa
• Gene Name: Homo sapiens SNF2 histone linker PHD RING helicase (SHPRH), transcript variant 1, mRNA.
• Database Link:
  NP_001036148
  Entrez Gene 257218 Human
  UniProt ID Q149N8
• Background: SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]). [supplied by OMIM]
• Synonyms: bA545I5.2

Reference Data

• Protein Families: Druggable Genome