UCK (UCK1) Mouse Monoclonal Antibody [Clone ID: OTI4C2]

CAT#: CF501473

Carrier-free (BSA/glycerol-free) UCK1 mouse monoclonal antibody, clone OTI4C2 (formerly 4C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCK1 (RC220876, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCK1. Positive lysates LY403114 (100ug) and LC403114 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UCK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-UCK1 mouse monoclonal antibody (TA501473) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UCK1 (RC220876).

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  HEK293T cells transfected with either RC220876 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UCK1 antibody (TA501473), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4C2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, Flow: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UCK1 (NP_113620) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.3 kDa
• Gene Name: Homo sapiens uridine-cytidine kinase 1 (UCK1), transcript variant 1, mRNA.
• Database Link:
  NP_113620
  Entrez Gene 83549 Human
  UniProt ID Q9HA47
• Background: Uridine/cytidine kinase-1 (EC 2.7.1.48) is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP) (Van Rompay et al., 2001 [PubMed 11306702]). [supplied by OMIM]
• Synonyms: URK1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism