TCTN2 Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: CF501477

Carrier-free (BSA/glycerol-free) TCTN2 mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCTN2 (RC203860, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TCTN2. Positive lysates LY411064 (100ug) and LC411064 (20ug) can be purchased separately from OriGene.

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  Anti-TCTN2 mouse monoclonal antibody (TA501477) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TCTN2 (RC203860).

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  HEK293T cells transfected with either RC203860 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TCTN2 antibody (TA501477), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1C1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TCTN2 (NP_079085) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 76.7 kDa
• Gene Name: Homo sapiens tectonic family member 2 (TCTN2), transcript variant 1, mRNA.
• Database Link:
  NP_079085
  Entrez Gene 79867 Human
  UniProt ID Q96GX1
• Synonyms: C12orf38; JBTS24; MKS8; TECT2