SH3 containing Grb 2 like 1 protein (SH3GL1) Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF501491

Carrier-free (BSA/glycerol-free) SH3GL1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3GL1 (RC201552, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3GL1. Positive lysates LY418946 (100ug) and LC418946 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SH3GL1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and SH3GL1-Knockout 293T cells (KO, Cat# LC812166) were separated by SDS-PAGE and immunoblotted with anti-SH3GL1 monoclonal antibody TA501491, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SH3GL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501491)

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  Anti-SH3GL1 mouse monoclonal antibody (TA501491) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3GL1 (RC201552).

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  Flow cytometric Analysis of Hela cells, using anti-SH3GL1 antibody (TA501491), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SH3GL1 antibody (TA501491), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2F5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SH3GL1 (NP_003016) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.3 kDa
• Gene Name: Homo sapiens SH3 domain containing GRB2 like 1, endophilin A2 (SH3GL1), transcript variant 1, mRNA.
• Database Link:
  NP_003016
  Entrez Gene 20405 MouseEntrez Gene 81922 RatEntrez Gene 6455 Human
  UniProt ID Q99961
• Synonyms: CNSA1; EEN; SH3D2B; SH3P8

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis