RIPPLY2 Mouse Monoclonal Antibody [Clone ID: OTI1B5]

CAT#: CF501512

Carrier-free (BSA/glycerol-free) RIPPLY2 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPPLY2 (RC220725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPPLY2. Positive lysates LY423172 (100ug) and LC423172 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC220725 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RIPPLY2 antibody (TA501512), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-RIPPLY2 antibody (TA501512), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RIPPLY2 (NP_001009994) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.7 kDa
• Gene Name: Homo sapiens ripply transcriptional repressor 2 (RIPPLY2), transcript variant 1, mRNA.
• Database Link:
  NP_001009994
  Entrez Gene 134701 Human
  UniProt ID Q5TAB7
• Synonyms: C6orf159; dJ237I15.1; SCDO6