QPRT Mouse Monoclonal Antibody [Clone ID: OTI4B4]

CAT#: CF501523

Carrier-free (BSA/glycerol-free) QPRT mouse monoclonal antibody, clone OTI4B4 (formerly 4B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY QPRT (RC202960, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-QPRT. Positive lysates LY402307 (100ug) and LC402307 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-QPRT monoclonal antibody.

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  HEK293T cells transfected with either RC202960 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-QPRT antibody (TA501523), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4B4
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human QPRT (NP_055113) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.6 kDa
• Gene Name: Homo sapiens quinolinate phosphoribosyltransferase (QPRT), transcript variant 1, mRNA.
• Database Link:
  NP_055113
  Entrez Gene 23475 Human
  UniProt ID Q15274
• Background: This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HEL-S-90n; QPRTase

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism