CORO1B Mouse Monoclonal Antibody [Clone ID: OTI3D10]

CAT#: CF501573

Carrier-free (BSA/glycerol-free) CORO1B mouse monoclonal antibody, clone OTI3D10 (formerly 3D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CORO1B (RC203023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1B. Positive lysates LY402787 (100ug) and LC402787 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CORO1B monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CORO1B monoclonal antibody (1:200).

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  Anti-CORO1B mouse monoclonal antibody (TA501573) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CORO1B (RC203023).

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  HEK293T cells transfected with either RC203023 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CORO1B antibody (TA501573), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3D10
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CORO1B (NP_065174) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.1 kDa
• Gene Name: Homo sapiens coronin 1B (CORO1B), transcript variant 1, mRNA.
• Database Link:
  NP_065174
  Entrez Gene 23789 MouseEntrez Gene 29474 RatEntrez Gene 57175 Human
  UniProt ID Q9BR76
• Background: Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 (PubMed 16027158)).(supplied by OMIM)Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signaltransduction (By similarity).
• Synonyms: CORONIN-2