KLHL2 Mouse Monoclonal Antibody [Clone ID: OTI10C12]

CAT#: CF501618

Carrier-free (BSA/glycerol-free) KLHL2 mouse monoclonal antibody, clone OTI10C12 (formerly 10C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (RC205347, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2. Positive lysates LY402119 (100ug) and LC402119 (20ug) can be purchased separately from OriGene.

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  Anti-KLHL2 mouse monoclonal antibody (TA501618) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2 (RC205347).

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  HEK293T cells transfected with either RC205347 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody (TA501618), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI10C12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65.8 kDa
• Gene Name: Homo sapiens kelch like family member 2 (KLHL2), transcript variant 1, mRNA.
• Database Link:
  NP_009177
  Entrez Gene 77113 MouseEntrez Gene 290692 RatEntrez Gene 11275 Human
  UniProt ID O95198
• Synonyms: ABP-KELCH; MAV; MAYVEN