FSH beta (FSHB) Mouse Monoclonal Antibody [Clone ID: OTI4E10]

CAT#: CF501623

Carrier-free (BSA/glycerol-free) FSHB mouse monoclonal antibody, clone OTI4E10 (formerly 4E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FSHB (RC214616, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FSHB. Positive lysates LY400397 (100ug) and LC400397 (20ug) can be purchased separately from OriGene.

• 

  HEK293T cells transfected with either RC214616 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FSHB antibody (TA501623), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4E10
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FSHB (NP_001018090) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.4 kDa
• Gene Name: Homo sapiens follicle stimulating hormone subunit beta (FSHB), transcript variant 2, mRNA.
• Database Link:
  NP_001018090
  Entrez Gene 2488 Human
  UniProt ID P01225
• Background: The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq]
• Synonyms: HH24

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: GnRH signaling pathway, Neuroactive ligand-receptor interaction