EPHX2 Mouse Monoclonal Antibody [Clone ID: OTI1A6]

CAT#: CF501626

Carrier-free (BSA/glycerol-free) EPXH2 mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (RC202489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2. Positive lysates LY419612 (100ug) and LC419612 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-EPHX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501626)

• Anti-EPHX2 mouse monoclonal antibody (TA501626) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2 (RC202489).

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Specifications

Product Data

• Clone Name: OTI1A6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.4 kDa
• Gene Name: Homo sapiens epoxide hydrolase 2 (EPHX2), transcript variant 1, mRNA.
• Database Link:
  NP_001970
  Entrez Gene 2053 Human
  UniProt ID P34913
• Background: This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]
• Synonyms: CEH; SEH

Reference Data

• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways