Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF501630

Carrier-free (BSA/glycerol-free) DYNC1LI1 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DYNC1LI1 (RC222010, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DYNC1LI1. Positive lysates LY414168 (100ug) and LC414168 (20ug) can be purchased separately from OriGene.

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  Anti-DYNC1LI1 mouse monoclonal antibody (TA501630) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DYNC1LI1 (RC222010).

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  HEK293T cells transfected with either RC222010 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DYNC1LI1 antibody (TA501630), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1C5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DYNC1LI1 (NP_057225) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.4 kDa
• Gene Name: Homo sapiens dynein cytoplasmic 1 light intermediate chain 1 (DYNC1LI1), transcript variant 1, mRNA.
• Database Link:
  NP_057225
  Entrez Gene 235661 MouseEntrez Gene 252902 RatEntrez Gene 51143 Human
  UniProt ID Q9Y6G9
• Background: Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
• Synonyms: DLC-A; DNCLI1; LIC1