Adenylate kinase 5 (AK5) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF501649

Carrier-free (BSA/glycerol-free) AK5 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (RC222241, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5. Positive lysates LY403569 (100ug) and LC403569 (20ug) can be purchased separately from OriGene.

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  Anti-AK5 mouse monoclonal antibody (TA501649) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK5 (RC222241).

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  HEK293T cells transfected with either RC222241 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK5 antibody (TA501649), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AK5 (NP_777283) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.2 kDa
• Gene Name: Homo sapiens adenylate kinase 5 (AK5), transcript variant 1, mRNA.
• Database Link:
  NP_777283
  Entrez Gene 229949 MouseEntrez Gene 26289 Human
  UniProt ID Q9Y6K8
• Background: This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: AK6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism