Breast cancer suppressor candidate 1 (VWA5A) Mouse Monoclonal Antibody [Clone ID: OTI3B4]

CAT#: CF501654

Carrier-free (BSA/glycerol-free) VWA5A mouse monoclonal antibody, clone OTI3B4 (formerly 3B4)

Product Images

Anti-VWA5A mouse monoclonal antibody (TA501654) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A (RC212185).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A. Positive lysates LY402354 (100ug) and LC402354 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC212185 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VWA5A antibody (TA501654), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-VWA5A antibody (TA501654), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-VWA5A antibody (TA501654), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3B4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VWA5A (NP_055437) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.3 kDa
• Gene Name: Homo sapiens von Willebrand factor A domain containing 5A (VWA5A), transcript variant 1, mRNA.
• Database Link:
  NP_055437
  Entrez Gene 4013 Human
  UniProt ID O00534
• Synonyms: BCSC-1; BCSC1; LOH11CR2A