IVD Mouse Monoclonal Antibody [Clone ID: OTI1F1]

CAT#: CF501670

Carrier-free (BSA/glycerol-free) IVD mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IVD (RC229217, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IVD. Positive lysates LY432239 (100ug) and LC432239 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC201077 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IVD antibody (TA501670), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1F1
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IVD (NP_002612) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.5 kDa
• Gene Name: Homo sapiens isovaleryl-CoA dehydrogenase (IVD), transcript variant 1, mRNA.
• Database Link:
  NP_002216
  Entrez Gene 24513 RatEntrez Gene 56357 MouseEntrez Gene 3712 Human
  UniProt ID P26440
• Background: Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: ACAD2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation