Haptoglobin (HP) Mouse Monoclonal Antibody [Clone ID: OTI1B1]

CAT#: CF501700

Carrier-free (BSA/glycerol-free) HP mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (RC223612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP. Positive lysates LY401576 (100ug) and LC401576 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HP monoclonal antibody.

• 

  HEK293T cells transfected with either RC223612 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HP antibody (TA501700), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1B1
• Applications: FC, WB
• Recommended Dilution: WB 1:200~1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.3 kDa
• Gene Name: Homo sapiens haptoglobin (HP), transcript variant 1, mRNA.
• Database Link:
  NP_005134
  Entrez Gene 3240 Human
  UniProt ID P00738
• Background: This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: BP; HP2ALPHA2; HPA1S

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane