DNAJA2 Mouse Monoclonal Antibody [Clone ID: OTI3A1]

CAT#: CF501711

Carrier-free (BSA/glycerol-free) DNAJA2 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJA2 (RC202204, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJA2. Positive lysates LY401779 (100ug) and LC401779 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJA2 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DNAJA2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501711, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501711, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DNAJA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501711, Dilution 1:50)

• Anti-DNAJA2 mouse monoclonal antibody (TA501711) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJA2 (RC202204).

• HEK293T cells transfected with either RC202204 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJA2 antibody (TA501711), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3A1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNAJA2 (NP_005871) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.6 kDa
• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member A2 (DNAJA2), mRNA.
• Database Link:
  NP_005871
  Entrez Gene 84026 RatEntrez Gene 478143 DogEntrez Gene 716353 MonkeyEntrez Gene 10294 Human
  UniProt ID O60884
• Background: The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq]
• Synonyms: CPR3; DJ3; DJA2; DNAJ; DNJ3; HIRIP4; PRO3015; RDJ2