TUBA3E Mouse Monoclonal Antibody [Clone ID: OTI10B4]

CAT#: CF501715

Carrier-free (BSA/glycerol-free) TUBA3E mouse monoclonal antibody, clone OTI10B4 (formerly 10B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA3E (RC209279, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA3E. Positive lysates LY404091 (100ug) and LC404091 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC209279 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TUBA3E antibody (TA501715), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI10B4
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TUBA3E (NP_997195) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.7 kDa
• Gene Name: Homo sapiens tubulin alpha 3e (TUBA3E), mRNA.
• Database Link:
  NP_997195
  Entrez Gene 112714 Human
  UniProt ID Q6PEY2
• Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).
• Synonyms: alpha 3e; tubulin

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection