KIST (UHMK1) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF501719

Carrier-free (BSA/glycerol-free) UHMK1 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UHMK1 (RC214962, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UHMK1. Positive lysates LY406187 (100ug) and LC406187 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC214962 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UHMK1 antibody (TA501719), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-UHMK1 antibody (TA501719), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-UHMK1 antibody (TA501719), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UHMK1 (NP_787062) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.4 kDa
• Gene Name: Homo sapiens U2AF homology motif kinase 1 (UHMK1), transcript variant 1, mRNA.
• Database Link:
  NP_787062
  Entrez Gene 246332 RatEntrez Gene 127933 Human
  UniProt ID Q8TAS1
• Background: The gene encodes a serine/threonine protein kinase that promotes cell cycle progression through G1 by phosphorylation of the cyclin-dependent kinase inhibitor 1B (p27Kip1), which causes nuclear export and degradation. The encoded protein is also thought to function in the adult nervous system and the gene has been associated with schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: KIS; KIST; P-CIP2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase