ARHGAP25 Mouse Monoclonal Antibody [Clone ID: OTI3G1]

CAT#: CF501749

Carrier-free (BSA/glycerol-free) ARHGAP25 mouse monoclonal antibody, clone OTI3G1 (formerly 3G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (RC217414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25. Positive lysates LY402385 (100ug) and LC402385 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501749, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501749, Dilution 1:50)

• 

  Anti-ARHGAP25 mouse monoclonal antibody (TA501749) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25 (RC217414).

• 

  HEK293T cells transfected with either RC217414 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGAP25 antibody (TA501749), and then analyzed by flow cytometry.

Show all 6 images

Specifications

Product Data

• Clone Name: OTI3G1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.3 kDa
• Gene Name: Homo sapiens Rho GTPase activating protein 25 (ARHGAP25), transcript variant 2, mRNA.
• Database Link:
  NP_055697
  Entrez Gene 232201 MouseEntrez Gene 500246 RatEntrez Gene 9938 Human
  UniProt ID P42331
• Background: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM]
• Synonyms: HEL-S-308; KAIA0053