PIK3AP1 Mouse Monoclonal Antibody [Clone ID: OTI7G9]

CAT#: CF501762

Carrier-free (BSA/glycerol-free) PIK3AP1 mouse monoclonal antibody, clone OTI7G9 (formerly 7G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (RC214125, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1. Positive lysates LY403461 (100ug) and LC403461 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PIK3AP1 monoclonal antibody.

• 

  Anti-PIK3AP1 mouse monoclonal antibody (TA501762) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIK3AP1 (RC214125).

• 

  HEK293T cells transfected with either RC214125 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIK3AP1 antibody (TA501762), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7G9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PIK3AP1 (NP_689552) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90.2 kDa
• Gene Name: Homo sapiens phosphoinositide-3-kinase adaptor protein 1 (PIK3AP1), mRNA.
• Database Link:
  NP_689522
  Entrez Gene 294048 RatEntrez Gene 608653 DogEntrez Gene 705017 MonkeyEntrez Gene 118788 Human
  UniProt ID Q6ZUJ8
• Background: Involved in the activation of phosphoinositide 3-kinase (PI3K) in B-cells and in natural killer (NK) cells. Couples B-cell antigen receptor (BCR) to PI3K activation by providing a docking site for the PI3K subunit PIK3R1, which contributes to B-cell development. Seems to have a complementary role with CD19 in PI3K activation (By similarity). May be involved in the survival of mature B cells via activation of REL (By similarity).
• Synonyms: BCAP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway