RND1 Mouse Monoclonal Antibody [Clone ID: OTI4F12]

CAT#: CF501769

Carrier-free (BSA/glycerol-free) RND1 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RND1 (RC205535, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RND1. Positive lysates LY415252 (100ug) and LC415252 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 1 cell line by using anti-RND1 monoclonal antibody (1:200).

• 

  Western blot analysis of extracts (10ug) from Hela cell line by using anti-RND1 monoclonal antibody (1:200).

• 

  HEK293T cells transfected with either RC205535 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RND1 antibody (TA501769), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4F12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RND1 (NP_055285) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.9 kDa
• Gene Name: Homo sapiens Rho family GTPase 1 (RND1), mRNA.
• Database Link:
  NP_055285
  Entrez Gene 27289 Human
  UniProt ID Q92730
• Background: Members of the Rho GTPase family, such as RND1, regulate the organization of the actin cytoskeleton in response to extracellular growth factors (Nobes et al., 1998 [PubMed 9531558]). [supplied by OMIM]
• Synonyms: ARHS; RHO6; RHOS

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Axon guidance