PECR Mouse Monoclonal Antibody [Clone ID: OTI1F2]

CAT#: CF501808

Carrier-free (BSA/glycerol-free) PECR mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECR (RC200822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECR. Positive lysates LY413023 (100ug) and LC413023 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-PECR monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501808)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501808)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501808)

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  Anti-PECR mouse monoclonal antibody (TA501808) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECR (RC200822).

• HEK293T cells transfected with either RC200822 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PECR antibody (TA501808), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1F2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PECR(NP_060911) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.4 kDa
• Gene Name: Homo sapiens peroxisomal trans-2-enoyl-CoA reductase (PECR), mRNA.
• Database Link:
  NP_060911
  Entrez Gene 55825 Human
  UniProt ID Q9BY49
• Synonyms: DCRRP; HPDHASE; HSA250303; PVIARL; SDR29C1; TERP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Biosynthesis of unsaturated fatty acids